As one of the major abiotic stresses, low temperature stress seriously hinders plant growth and negatively affects the yield and quality of crops. 5-Aminolevulinic acid (ALA) is a growth regulator widely present in animals and plants. Due to its high efficiency, non-toxicity and easy degradation, it is widely used in the process of cold tolerance of plants.
However, most current research related to ALA mainly focuses on regulating network endpoints. The specific molecular mechanism of ALA action in early cold tolerance of plants is currently unclear and requires further research by scientists.
In January 2024, Horticultural Research published a research paper entitled “5-Aminolevulinic Acid Enhances Cold Tolerance by Regulating the SlMYB4/SlMYB88-SlGSTU43 Reactive Oxygen Species Scavenging Module in Tomato” by Hu Xiaohui’s team at Northwestern University agriculture and forestry.
In this study, the glutathione S-transferase gene SlGSTU43 was identified in tomato (Solanum lycopersicum L.). The results of the study showed that ALA strongly induces the expression of SlGSTU43 under cold stress. Transgenic tomato lines overexpressing SlGSTU43 exhibited significantly increased reactive oxygen species scavenging capacity and showed significant resistance to low temperature stress, whereas SlGSTU43 mutant lines were sensitive to low temperature stress.
In addition, the research results showed that ALA does not increase the tolerance of the mutant strain to low temperature stress. Thus, the study suggests that SlGSTU43 is an important gene in the process of enhancing cold tolerance in tomato by ALA (Fig. 1).
In addition, this study confirmed through EMSA, Y1H, LUC and ChIP-qPCR detection that SlMYB4 and SlMYB88 can regulate the expression of SlGSTU43 by binding to the SlGSTU43 promoter. Further experiments showed that SlMYB4 and SlMYB88 are also involved in the ALC process by increasing tomato tolerance to low temperature stress and positively regulating the expression of SlGSTU43 (Fig. 2). These results provide new insight into the mechanism by which ALA enhances tolerance to low temperature stress in tomato.
Further information: Zhengda Zhang et al., 5-aminolevulinic acid enhances cold tolerance by regulating the SlMYB4/SlMYB88-SlGSTU43 module for reactive oxygen species scavenging in tomato, Horticulture Research (2024). DOI: 10.1093/hour/uhae026
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Post time: Jul-22-2024